Test Methods and Protocols for SILVADUR™

The following four test methods are currently approved for evaluating application
efficiency, technology performance, and wash durability for SILVADUR™ treated fabrics.

1. Colorimetric Test for Rapid Identification of SILVADUR™ in Textiles
2. Antimicrobial Test Method for Antibacterial Performance:
   ASTM E3160-18 or equivalent such as AATCC 100 – 2019 test method
3. Performance Durability by Washing Methods: ASTM E3162-18 or
   AATCC 61-2A
4. Odor Control Sensory Test Method IACM 0710: Biofouling of textiles
   from milk

When requesting performance evaluation from Third Party Facilities, it is very
important that the exact specifications and requirements are followed. A minimum of
20 grams each of SILVADUR™ treated and untreated fabrics are required to complete
the evaluations.

1. Colorimetric Test for Rapid Identification of SILVADUR™ in Textiles

This on-site qualitative method can be used to detect silver in fabric of any color and composition. Detection is performed using a
commercially available reagent kit.

Picture 1 : no SILVADUR™

Picture 2 : with SILVADUR™

This analysis is fast, qualitative, and reproducible. This method can be used to ensure the presence of SILVADUR™ product in
fabrics down to approximately 10 ppm silver.

2. Antimicrobial test methods for antibacterial performance: ASTM E3160 – 2018 and AATCC 100-2019

Real life activities may change the balance of one’s microbiome and produce more microorganisms that cause malodor. Controlling
the presence of microorganisms is one way to control the generation of malodor. This antimicrobial evaluation measures whether a
SILVADUR™ treated fabric can directly eradicate or reduce bacteria or inhibit their growth when compared to untreated fabric.
ASTM E3160 – 2018 and AATCC 100-2019 are the two standard methods that have been validated for consistency and reproducibility for
SILVADUR™ treated fabrics.

 

3. Performance durability by washing methods: ASTM E3162-18 or AATCC 61-2A

Example of Antimicrobial Test Results performed using ASTM E3160 – 2018 test method

The tested samples below demonstrated significant antimicrobial activity performance after multiple laundering cycles.

Type of material	100% Polyester
Antimicrobial agent	3% SILVADUR™ 960Flex
Application Process	Padding

Testing Parameters

Method	ASTM E3160
Microorganism	Microorganism Escherichia coli (ATCC # 25922)
Amount of test sample	0.4 gram
Contact time	24 hours
Initial bacterial inoculum	E. coli 2.36E+05
Nutrient dilution in inoaculum	1:20
Wash protocol	ASTM E3162-18

Testing Results

Sample ID	Bacterial reduction from supplied control E. coli
Control- untreated after 40 washes	–
Silvadur Treated After 40 washes (8 cycles)	>99.9%

4. Odor Sensory Test Method IACM 0710: Biofouling of textiles from milk

Example of the odor evaluation
Test Method: IACM 0710: Qualitative Evaluation for Odor/Stain Resistance of Antimicrobial Treated Articles

Testing Results

IAC #	Sample Description	24 Hrs. Rating	48 Hrs. Rating
5698-1	SILVADUR™ treated sample after 40 washes (ASTM E3162-18 – 8 cycles)	1	1
5698-2	Untreated sample	2.3	3.7
	IAC Standard Untreated Control	3	5